Anaerobic Growth and Maintenance of Mammalian Cell Lines

Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.

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Bibliographic Details
Main Author: Plotkin, Balbina J.
Format: Electronic Video
Language:English
Published: Cambridge, MA MyJoVE Corp 2016
Series:Biology
Subjects:
Online Access:CONNECT