Substrate Generation for Endonucleases of CRISPR/Cas Systems
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclea...
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
|
| Series: | Biology
|
| Subjects: | |
| Online Access: | CONNECT |
