A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
We developed a quantitative DNA-binding, ELISA-based assay to measure transcription factor interactions with DNA. High specificity for the RUNX2 protein was achieved with a consensus DNA-recognition oligonucleotide and specific monoclonal antibody. Colorimetric detection with an enzyme-coupled anti...
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| Main Author: | |
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| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
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| Series: | Biology
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| Subjects: | |
| Online Access: | CONNECT |
