Biomedical electron microscopy : illustrated methods and interpretations /

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also e...

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Bibliographic Details
Main Author: Maunsbach, Arvid Bernhard
Other Authors: Afzelius, Björn
Format: Electronic eBook
Language:English
Published: San Diego, Calif. : Academic Press, ©1999.
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100 1 |a Maunsbach, Arvid Bernhard. 
245 1 0 |a Biomedical electron microscopy :  |b illustrated methods and interpretations /  |c Arvid B. Maunsbach, Björn A. Afzelius. 
260 |a San Diego, Calif. :  |b Academic Press,  |c ©1999. 
300 |a 1 online resource (xv, 548 pages) :  |b illustrations (some color) 
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504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
505 0 |a Preface. Acknowledgments. Micrograph Interpretation. Fixatives. Fixative Vehicle. Fixative Application. Dehydration and Embedding. Freezing and Freeze-Substitution. Support Films. Ultramicrotomy. Section-Staining. Microscopy. Image Recording. Photographic and Digital Printing. Negative Staining. Autoradiography. Cytochemistry. Immunocytochemistry. Freeze-Fracturing and Shadowing. Sampling and Quantitation. Image Processing. 3D Reconstructions. Appendix: Practical Methods. Index. Acknowledgment for Figures. 
520 |a This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Key Features * Authored by the key leaders in the biological electron microscopy field * Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines * Introduces students on how to read and interpret electron micrographs. 
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